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Multi‐omics atlas of combinatorial abiotic stress responses in wheat

Par Conseil national de recherches du Canada

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DOITrouver le DOI : https://doi.org/10.1111/tpj.16332
AuteurRechercher : 1, 2; Rechercher : 1; Rechercher : 3Identifiant ORCID : https://orcid.org/0000-0002-9345-9640; Rechercher : 3; Rechercher : 3; Rechercher : 1; Rechercher : 4; Rechercher : 1; Rechercher : 1; Rechercher : 5; Rechercher : 5; Rechercher : 1; Rechercher : 5Identifiant ORCID : https://orcid.org/0000-0001-5551-7232; Rechercher : 5Identifiant ORCID : https://orcid.org/0000-0002-2173-0876; Rechercher : 1Identifiant ORCID : https://orcid.org/0000-0002-7213-1590
Affiliation
  1. Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
  2. Summerland Research and Development Centre Agriculture and Agri‐Food Canada
  3. Saskatoon Research and Development Centre Agriculture and Agri‐Food Canada
  4. Department of Biological Sciences University of Calgary
  5. Department of Cell and Systems Biology University of Toronto
Bailleur de fondsRechercher : Natural Sciences and Engineering Research Council of Canada
FormatTexte, Article
Sujetwheat (Triticum aestivumL.); abiotic stress; multi-omics; multi-environmental stresses; RNAseq; physiological traits; transcription factors; Ethylene-responsive element binding factor-associ-ated amphiphilic repression motif
Résumé
Field-grown crops rarely experience growth conditions in which yield can be maximized. Environmental stresses occur in combination, with advancements in crop tolerance further complicated by its polygenic nature. Strategic targeting of causal genes is required to meet future crop production needs. Here, we employed a systems biology approach in wheat (Triticum aestivum L.) to investigate physio-metabolic adjustments and transcriptome reprogramming involved in acclimations to heat, drought, salinity and all combinations therein. A significant shift in magnitude and complexity of plant response was evident across stress scenarios based on the agronomic losses, increased proline concentrations and 8.7-fold increase in unique differentially expressed transcripts (DETs) observed under the triple stress condition. Transcriptome data from all stress treatments were assembled into an online, open access eFP browser for visualizing gene expression during abiotic stress. Weighted gene co-expression network analysis revealed 152 hub genes of which 32% contained the ethylene-responsive element binding factor-associated amphiphilic repression (EAR) transcriptional repression motif. Cross-referencing against the 31 DETs common to all stress treatments isolated TaWRKY33 as a leading candidate for greater plant tolerance to combinatorial stresses. Integration of our findings with available literature on gene functional characterization allowed us to further suggest flexible gene combinations for future adaptive gene stacking in wheat. Our approach demonstrates the strength of robust multi-omics-based data resources for gene discovery in complex environmental conditions. Accessibility of such datasets will promote cross-validation of candidate genes across studies and aid in accelerating causal gene validation for crop resiliency.
Date de publication
Maison d’éditionWiley
Licence
Dans
  • The Plant Journal (29 mai 2023).
Langueanglais
Publications évaluées par des pairsOui
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Identificateur de l’enregistrement46b45728-167a-4ae7-8938-26447d309ed4
Enregistrement créé2023-07-31
Enregistrement modifié2023-07-31
Date de modification :