Primary and secondary antibody responses to immunisation with single antigens and Aeromonas salmonicida bacterin in Atlantic salmon, Salmo salar L.

Par Conseil national de recherches du Canada

Auteur	Rechercher : Manning, A. J.¹; Rechercher : Dacanay, A.²; Rechercher : Johnson, S. C.²
Affiliation	Conseil national de recherches du Canada. Initiative en génomique et en santé du CNRC Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
Format	Texte, Allocution
Conférence	Aquaculture Canada 2007, 23-26 September 2007, Edmonton, Alberta, Canada
Résumé	An important property of an effective vaccine is its ability to establish immunological memory. In this 25 week study, we compared primary and secondary antibody responses of individually tagged juvenile salmon immunised with three non-adjuvanted preparations: i) recombinant TapA protein, an A. salmonicida pilus protein that is a putative thymus-dependent antigen with memory-inducing potential; ii) lipopolysaccharide (LPS) purified from A. salmonicida (A449), a thymus-independent antigen of poor memory-inducing potential; and, iii) bacterin, the basis of most commercial vaccines, a suspension of formalin-killed A. salmonicida (A449) cells cultured under ironrestricted conditions. ELISA results showed that primary antibody responses to rTapA or LPS were synchronized while bacterin responses were individually variable. Immunological memory followed secondary immunisation with rTapA, but not LPS, which suggests that thymus-dependent and thymus-independent designations apply to these antigens. The immune response of bacterin-immunised fish to A. salmonicida lacked evidence of memory and was dominated by antibodies produced against LPS. This suggests that bacterin-based vaccines, while capable of producing short-term immunity to A. salmonicida, may not be able to stimulate immunological memory for adequate long-term antigen recognition. The study highlights the need to ensure that bacterin preparations are supplemented with thymus-dependent antigens capable of stimulating immunological memory.
Date de publication	2008-01-01
Dans	Proceedings of Contributed Papers of the 24th Annual Meeting of the Aquaculture Association of Canada (1 janvier 2008).
Langue	anglais
Publications évaluées par des pairs	Non
Numéro du CNRC	NRCC 42765 NRC-IMB-1728
Numéro NPARC	3538069
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	46f10f17-8dad-45c9-8ea7-6d848c3861de
Enregistrement créé	2009-03-01
Enregistrement modifié	2020-03-03

Date de modification :: 2024-09-07