| DOI | Trouver le DOI : https://doi.org/10.1139/m71-019 |
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| Auteur | Rechercher : Oka, T.1; Rechercher : Simpson, F. J.1; Rechercher : Child, J. J.1; Rechercher : Mills, Sister Cecily1 |
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| Affiliation | - Conseil national de recherches du Canada. Laboratoire régional des prairies
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| Format | Texte, Article |
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| Résumé | Evidence is presented that a single enzyme, quercetinase, is responsible for the degradation of quercetin by Aspergillus flavus to yield carbon monoxide and a depside, 2-protocatechuoylphloroglucinol carboxylic acid. A procedure for the isolation of the dioxygenase as a homogeneous protein is described. The most purified preparation degraded 10 800 μmoles of quercetin/h mg protein and was homogeneous as judged by ultracentrifugation and by electrophoresis. The molecular weight was determined as 111 000 + 4000. Kₘ values for quercetin and oxygen as substrates were 5.2 × 10⁻⁶ M and 1.2 × 10⁻⁴ M respectively. The enzyme is a glycoprotein containing 27.5% carbohydrate and the amino acid composition is presented. |
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| Date de publication | 1971-01-01 |
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| Maison d’édition | Canadian Science Publishing |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | NRCC-11638 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 485846d8-d2f3-4934-b953-1d8179751a18 |
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| Enregistrement créé | 2023-09-13 |
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| Enregistrement modifié | 2023-09-13 |
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