| DOI | Trouver le DOI : https://doi.org/10.1016/j.jcis.2020.07.097 |
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| Auteur | Rechercher : Shrestha, Buddha R.; Rechercher : Liberelle, Benoit; Rechercher : Murschel, Frederic; Rechercher : Purisima, Enrico O.1; Rechercher : Sulea, Traian1; Rechercher : De Crescenzo, Gregory; Rechercher : Banquy, XavierIdentifiant ORCID : https://orcid.org/0000-0002-3342-3179 |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Bailleur de fonds | Rechercher : Canada Research Chairs |
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| Format | Texte, Article |
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| Sujet | coiled coils; surface forces; binding; folding; induced fit; energy landscape |
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| Résumé | We used the Surface Forces Apparatus to elucidate the interaction mechanism between grafted 5 heptad-long peptides engineered to spontaneously form a heterodimeric coiled-coil complex. The results demonstrated that when intimate contact between peptides is reached, binding occurs first via weakly interacting but more mobile distal heptads, suggesting an induced-fit association process. Precise control of the distance between peptide-coated surfaces allowed to quantitatively monitor the evolution of their biding energy. The binding energy of the coiled-coil complex increased in a stepwise fashion rather than monotonically with the overlapping distance, each step corresponding to the interaction between a quantized number of heptads. Surface forces data were corroborated to surface plasmon resonance measurements and molecular dynamics simulations and allowed the calculation of the energetic contribution of each heptad within the coiled-coil complex. |
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| Date de publication | 2020-07-23 |
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| Maison d’édition | Elsevier |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | S0021979720309796 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 4b6d4484-59b9-4356-8290-865aa8ec244a |
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| Enregistrement créé | 2022-09-23 |
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| Enregistrement modifié | 2022-09-23 |
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