| Auteur | Rechercher : High, N.; Rechercher : Deadman, M.; Rechercher : Hood, D.; Rechercher : Moxon, E. |
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| Format | Texte, Article |
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| Sujet | Antibodies,Monoclonal; Bacterial Proteins; Bacteriological Techniques; Base Sequence; Blotting,Southern; Chromosomes,Bacterial; DNA Transposable Elements; DNA,Bacterial; Enhancer Elements (Genetics); Escherichia coli; Escherichia-coli; Genes,Bacterial; Genetic Screening; Haemophilus; Haemophilus influenzae; Lipopolysaccharide; Lipopolysaccharides; Molecular Sequence Data; Mutagenesis; Plasmids; Polymerase Chain Reaction; Restriction Mapping; Sequence Analysis,DNA; Sequence Homology,Amino Acid; Support,Non-U.S.Gov't |
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| Résumé | Mutagenesis with the transposon Tn916 was used as a strategy to identify genes required for synthesis of the Gal alpha (1-4) beta Gal component of Haemophilus influenzae strain RM7004 lipopolysaccharide. Insertion of Tn916 into an open reading frame (ORF) encoding a protein with 75% homology to the Escherichia coli methionine related protein (Mrp) is described. Mutations in mrp resulted in loss of reactivity with monoclonal antibody (mAb) 4C4, which recognises Gal alpha (1-4) beta Gal, and expression of LPS with a different electrophoretic profile to that of wild-type RM7004. An unexpected feature of this mutation was that it appeared to influence the number of copies of 5'-CAAT-3' present in lic2A, a gene which is also required for biosynthesis and phase variable expression of the Gal alpha (1-4) beta Gal LPS epitope Type: JOURNAL ARTICLE Language: Eng |
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| Date de publication | 1996-12-15 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | HIGH1996 |
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| Numéro NPARC | 9389136 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 521d0779-8c67-4ecc-92bf-ba74284b4a8e |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2022-11-18 |
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