| DOI | Trouver le DOI : https://doi.org/10.1007/s10719-008-9172-2 |
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| Auteur | Rechercher : Sauerzapfe, Birgit; Rechercher : Krenek, Karel; Rechercher : Schmiedel, Judith; Rechercher : Wakarchuk, Warren W.1; Rechercher : Pelantová, Helena; Rechercher : Kren, Vladimir; Rechercher : Elling, Lothar |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | poly-LacNAc; chemo-enzymatic synthesis; galectin binding; ECM glycoproteins; biomaterials |
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| Résumé | Poly-N-acetyllactosamine (poly-LacNAc) structures have been identified as important ligands for galectin-mediated cell adhesion to extra-cellular matrix (ECM) proteins. We here present the biofunctionalization of surfaces with poly-LacNAc structures and subsequent binding of ECM glycoproteins. First, we synthesized β-GlcNAc glycosides carrying a linker for controlled coupling onto chemically functionalized surfaces. Then we produced poly-LacNAc structures with defined lengths using human β1,4-galactosyltransferase-1 and β1,3-N-acetylglucosaminyltransferase from Helicobacter pylori. These compounds were also used for kinetic characterization of glycosyltransferases and lectin binding assays. A mixture of poly-LacNAc-structures covalently coupled to functionalized microtiter plates were identified for best binding to our model galectin His6CGL2. We further demonstrate for the first time that these poly-LacNAc surfaces are suitable for further galectin-mediated binding of the ECM glycoproteins laminin and fibronectin. This new technology should facilitate cell adhesion to biofunctionalized surfaces by imitating the natural ECM microenvironment. |
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| Date de publication | 2008-09-28 |
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| Maison d’édition | Springer |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 9381535 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 537b7179-0fb6-48d3-ac48-3d42582300ab |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-04-15 |
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