Quercetin deformable liposome: Preparation and efficacy against ultraviolet B induced skin damages in vitro and in vivo

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1016/j.jphotobiol.2013.07.014
Auteur	Rechercher : Liu, D.; Rechercher : Hu, H.; Rechercher : Lin, Z.; Rechercher : Chen, D.; Rechercher : Zhu, Y.; Rechercher : Hou, S.¹; Rechercher : Shi, X.
Affiliation	Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
Format	Texte, Article
Sujet	3,4 methylenedioxyamphetamine; cholesterol; cholic acid; liposome; malonaldehyde; phosphatidylcholine; polysorbate 80; quercetin; reactive oxygen metabolite; sorbitan laurate; animal cell; animal experiment; cell membrane; cell protection; cell viability; dermatitis; drug delivery system; drug efficacy; drug formulation; drug release; drug solubility; drug synthesis; elasticity; encapsulation; histopathology; in vitro study; in vivo study; keratinocyte; lipid peroxidation; male; oxidative stress; particle size; rat; skin defect; skin edema; stratum corneum; ultraviolet B radiation; ultraviolet irradiation; zeta potential
Résumé	Ultraviolet (UV) radiation has deleterious effects on cells through direct damage to DNA or through increasing generation of reactive oxygen species (ROS). The flavonol quercetin (Qu) provides cellular protection against UV radiation and the current investigation was carried out to develop a deformable liposome formulation of Qu to enhance its delivery into human skin and to improve its anti-UVB effect. The influence of surfactants (including Span 20, Tween 80 and sodium cholate) on the properties of Qu deformable liposomes was investigated. Liposomes composed of Qu, phosphatidylcholine (PC), cholesterol (Chol), and Tween 80 showed high entrapment efficiencies (80.41 ± 4.22%), small particle sizes (132 ± 14 nm), high elasticity (10.48 ± 0.71), and prolonged drug release. The cell viability in UVB-irradiated HaCaT cells increased to 89.89 ± 4.5% at 24 h and 78.8 ± 3.19% at 48 h following treatment with Qu defomable liposomes. The ROS and malondialdehyde (MDA) level were also reduced. The penetration rate was 3.8-fold greater than that of the Qu suspension. Moreover, the edema and inflammation was alleviated by Qu deformable liposomes. These results showed the potential of deformable liposomes to enhance the anti-UVB effects of Qu both in vitro and in vivo. © 2013 Elsevier B.V. All rights reserved.
Date de publication	2013
Dans	Journal of Photochemistry and Photobiology B: Biology 127 (2013) : 8–17.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro NPARC	21269779
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	54320dd5-68fe-4e5b-99f7-abaf9344106d
Enregistrement créé	2013-12-13
Enregistrement modifié	2020-04-22

Date de modification :: 2024-06-29