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Structure of the capsular polysaccharides and lipopolysaccharides from Haemophilus parasuis strains ER-6P (serovar 15) and Nagasaki (serovar 5)

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.carres.2013.04.023
AuteurRechercher : 1; Rechercher : 1; Rechercher : ; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada
FormatTexte, Article
SujetCapsular polysaccharides; Capsule; Gram-negative bacteria; Haemophilus parasuis; Lipooligosaccharides; Lipopolysaccharides; Upper respiratory tract; Virulent strains; Bacteria; Chains; Mass spectrometry; Nuclear magnetic resonance spectroscopy; Polysaccharides; Strain; bacterial polysaccharide; bacterium lipopolysaccharide; dextro glycero dextro manno heptose; galactosamine; galactose; glucose; glycoprotein; l glycero dextro manno heptose; monosaccharide; n glycoloylneuraminic acid; neuraminic acid; sephadex; unclassified drug; bacterial cell; bacterial strain; chemical structure; dephosphorylation; gel chromatography; Haemophilus parasuis; mass spectrometry; molecular mimicry; nuclear magnetic resonance spectroscopy; polyacrylamide gel electrophoresis; proton nuclear magnetic resonance; serotype; terminal sequence; Animalia; Bacteria (microorganisms); Haemophilus parasuis; Negibacteria; Pasteurellaceae; Suidae
Résumé
Haemophilus parasuis is a Gram-negative bacterium from the family Pasteurellaceae and a swine pathogen. H. parasuis is found in the upper respiratory tract of piglets and produces Glässer's disease, an invasive disease characterized by polyserositis. H. parasuis contains a short lipopolysaccharide (LPS) or lipooligosaccharide (LOS) reported to play a partial role in interaction with host cells. The presence of capsule has been phenotypically demonstrated in certain H. parasuis strains and its role in virulence has been suggested, but the chemical structure of the surface polysaccharides of this bacterium was unknown. The structure of capsular polysaccharide (CPS) and LOS from virulent strains ER-6P and Nagasaki was studied by NMR spectroscopy, mass spectrometry and chemical methods. CPS from both strains had the same main chain with disaccharide repeating unit, substituted with α-Neu5R-(2-3)-α-GalNAc-(1-P-(strain ER-6P) or α-Neu5R-(2-3)-α-Gal-(1-P-strain Nagasaki) side chains, where R is the N-acetyl or N-glycolyl group. Glycolyl-neuraminic acid is widely found in animal glycoproteins, but it apparently has not been found in bacteria before, and might be important for the biology of this microorganism. Ac and Gc were present in equal amounts in the strain ER-6P but Nagasaki contained only about 20% of Gc substituent. Both strains produced the same LPS of a rough type with a single phosphorylated Kdo linking core and lipid A parts. LOS structure was similar to some strains of H. influenzae and contained a globotetraose terminal sequence. Crown Copyright © 2013 Published by Elsevier Ltd.
Date de publication
Dans
  • Carbohydrate Research 378 (2013) : 91–97.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21270551
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Identificateur de l’enregistrement5606ea52-980a-4370-a6f4-25da606052b2
Enregistrement créé2014-02-17
Enregistrement modifié2020-04-22
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