Sélection de la langue

Gouvernement du Canada / Government of Canada

Recherche

Genome-wide transcriptional profiling and enrichment mapping reveal divergent and conserved roles of Sko1 in the Candida albicans osmotic stress response

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.ygeno.2013.06.002
AuteurRechercher : ; Rechercher : 1; Rechercher : ; Rechercher : ; Rechercher :
Affiliation
  1. Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
FormatTexte, Article
Sujetphosphotransferase; protein hog1; unclassified drug; Candida albicans; computer model; DNA binding motif; enzyme activity; gene; gene function; gene mapping; genetic conservation; genetic regulation; genetic transcription; genetic variability; genome analysis; microarray analysis; osmotic stress; real time polymerase chain reaction; signal transduction; Sko1 gene; Candida albicans; Saccharomyces cerevisiae
Résumé
Candida albicans maintains both commensal and pathogenic states in humans. Here, we have defined the genomic response to osmotic stress mediated by transcription factor Sko1. We performed microarray analysis of a sko1δ/δ mutant strain subjected to osmotic stress, and we utilized gene sequence enrichment analysis and enrichment mapping to identify Sko1-dependent osmotic stress-response genes. We found that Sko1 regulates distinct gene classes with functions in ribosomal synthesis, mitochondrial function, and vacuolar transport. Our in silico analysis suggests that Sko1 may recognize two unique DNA binding motifs. Our C. albicans genomic analyses and complementation studies in Saccharomyces cerevisiae showed that Sko1 is conserved as a regulator of carbohydrate metabolism, redox metabolism, and glycerol synthesis. Further, our real time-qPCR results showed that osmotic stress-response genes that are dependent on the kinase Hog1 also require Sko1 for full expression. Our findings reveal divergent and conserved aspects of Sko1-dependent osmotic stress signaling. © 2013 Elsevier Inc.
Date de publication
Dans
  • Genomics 102, nº 4 (2013) : 363–371.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21269970
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement5e2639a2-199a-426f-9079-e1d8c282ffa5
Enregistrement créé2013-12-13
Enregistrement modifié2020-04-22
Date de modification :