| DOI | Trouver le DOI : https://doi.org/10.1117/12.567386 |
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| Auteur | Rechercher : Tay, Li-Lin1; Rechercher : Rowell, Nelson L.1; Rechercher : Lockwood, David J.1; Rechercher : Boukherroub, Rabah |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences des microstructures du CNRC
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| Format | Texte, Article |
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| Conférence | Photonics North 2004: Photonic Alications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education, 27 September 2004, Ottawa, Canada |
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| Résumé | The large surface area within porous Si (pSi) and its strong room temperature photoluminescence (PL) make it an ideal host for biological sensors. In particular, the development of pSi-based optical sensors for DNA, enzyme and other biochemical molecules have become of great interest. Here, we demonstrate that the in-situ monitoring of the pSi PL behaviour can be used as a positive identification of bovine serum albumin (BSA) protein adsorption inside the porous matrix. Electrochemically prepared pSi films were first functionalized with undecylenic acid to produce an organic monolayer covalently attached to the porous silicon surfaces. The acid terminal group also provided favourable BSA binding sites on the pSi matrix sidewalls. In-situ PL spectra showed a gradual red shift (up to 12 meV) in the PL peak energy due to the protein incorporation into the porous matrix. The PL then exhibited a continuous blue shift after saturation of the protein molecules in the pores. This blue shift of the PL peak frequency and a steady increase in the PL intensity is evidence of surface oxidation. Comparing the specular reflectance obtained by Fourier transform infrared spectroscopy (FTIR) before and after BSA incubation confirmed the adsorption of protein in the pSi matrix. |
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| Date de publication | 2004 |
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| Dans | |
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| Série | |
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| Langue | anglais |
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| Numéro du CNRC | NRC-INMS-1392 |
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| Numéro NPARC | 12346627 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 60b997dd-85ee-4af6-bdef-b22047915597 |
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| Enregistrement créé | 2009-09-17 |
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| Enregistrement modifié | 2020-04-17 |
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