Téléchargement | - Voir le manuscrit accepté : Near-field optical probes provide subdiffraction-limited excitation areas for fluorescence correlation spectroscopy on membranes (PDF, 918 Kio)
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DOI | Trouver le DOI : https://doi.org/10.1351/PAC-CON-08-10-10 |
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Auteur | Rechercher : Vobornik, Dusan1; Rechercher : Banks, Daniel S.; Rechercher : Lu, Zhengfang1; Rechercher : Fradin, Cécile; Rechercher : Taylor, Rod1; Rechercher : Johnston, Linda J.1 |
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Affiliation | - Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
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Format | Texte, Article |
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Sujet | bilayers; fluorescence correlation spectroscopy; near-field scanning optical microscopy; single-molecule dynamics |
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Résumé | Near-field optical probes have been used to produce a subdiffraction-limited observation area for fluorescence correlation spectroscopy (FCS) experiments on supported membranes. The design of a bent, etched fiber probe that is compatible with biological imaging in an aqueous environment is described. This probe design is used for proof of principle experiments to measure lipid diffusion in a fluid-supported bilayer. A reduction in excitation area of approximately one order of magnitude (relative to a confocal FCS experiment) is obtained with a probe aperture diameter of 140 nm. We also demonstrate a simple approach for modeling the autocorrelation decay due to diffusion within the excitation profile at the near-field scanning optical microscopy (NSOM) probe aperture. The use of probes with smaller apertures is expected to provide an additional order of magnitude reduction in the observation area, thus enabling the study of cellular membranes with higher concentrations of fluorophores than is currently possible with diffraction-limited techniques. |
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Date de publication | 2009 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 14829320 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | 60d356ac-e42c-4697-ac4d-200112b4f8c0 |
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Enregistrement créé | 2011-02-15 |
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Enregistrement modifié | 2020-04-16 |
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