| DOI | Trouver le DOI : https://doi.org/10.1002/(SICI)1521-379X(199904)51:4<111::AID-STAR111>3.0.CO;2-A |
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| Auteur | Rechercher : Båga, Monica1; Rechercher : Repellin, Anne1; Rechercher : Demeke, Tigst1; Rechercher : Caswell, Karen1; Rechercher : Leung, Nick1; Rechercher : Chibbar, Ravind N.1; Rechercher : Abdel-Aal, El-Sayed; Rechercher : Hucl, Pierre |
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| Affiliation | - Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
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| Format | Texte, Article |
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| Conférence | Presented at the 49th Starch Convention, April 22, 1998, Detmold, Germany |
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| Résumé | Natural mutations that affect the amylose/amylopectin ratio in starch are unlikely to develop naturally in wheat due to its allohexaploid genome (2n=6x; AABBDD). One of the strategies to modify wheat starch structure involves identification of germplasms with null alleles for starch biosynthetic genes, followed by exchange of functional alleles with the identified null alleles through classical plant breeding. This technique has successfully been used to combine the three null alleles for granule‐bound starch synthase I (GBSSI) to develop a wheat line that produces amylopectin‐rich (>95%) starch (waxy starch). Another strategy to alter expression levels of starch biosynthetic genes employs recent advances in molecular biology and genetic engineering of wheat. For this approach, various monocot vectors have been developed that drive expression of wheat starch branching enzyme I (SBEI) cDNA sequences in the anti‐sense orientation. Several of the wheat cell lines transformed with the anti‐sense vectors express branching enzyme (BE) activity at a significantly lower level than non‐transformed cells. One transgenic wheat plant expressing the anti‐sense SBEI RNA produces a ten‐fold lower level of BE activity in kernels than wild‐type wheat. Analysis of starch produced from the transgenic plant shows that starch structure and properties have been altered. |
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| Date de publication | 1999-04 |
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| Maison d’édition | Wiley |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC-EME-42617 |
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| Numéro NPARC | 13052810 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 61ad23d2-506a-4cf8-afe2-20e47dbb1ff5 |
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| Enregistrement créé | 2009-11-25 |
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| Enregistrement modifié | 2022-03-10 |
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