| DOI | Trouver le DOI : https://doi.org/10.1194/jlr.D900013-JLR200 |
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| Auteur | Rechercher : Zhou, Ping1; Rechercher : Chandan, Vandana1; Rechercher : Liu, Xin1; Rechercher : Chan, Kenneth1; Rechercher : Altman, Eleonora1; Rechercher : Li, Jianjun1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | Helicobacter pylori; acylation; microwave-assisted digestion; mass spectrometry; MALDI-TOF/TOF |
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| Résumé | The lipid A of Gram-negative bacteria plays a major role in the pathogenesis of bacterial infections. Lipid A diversity is observed both in the number and length of fatty acid side chains and in the presence of terminal phosphate residues and associated modifications. In this report, we describe a new sample preparation method based on microwave-assisted enzymatic digestion and detergent-free mild hydrolysis, in conjunction with a MALDI-time-of-flight (TOF)/TOF analysis, to determine the structures of lipid A from Helicobacter pylori . The total time for sample preparation and mass spectrometric analysis is within 2 h and applicable to profiling the lipid A structures from dried bacterial cells on as little as 1 µg. The reliability of the technique was further demonstrated through the analysis of the lipid A from bacterial cells of different H. pylori strains. The phosphorylation and acylation patterns of lipid A could be elucidated using material from a single colony. Furthermore, we found unusual heptaacyl lipid A species present in H. pylori mutant that have not been previously reported, although the abundance was relatively low. The present study provides the first characterization of the lipid A component from a single bacterial colony sample by mass spectrometry. |
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| Date de publication | 2009-09 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 15329308 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 625f2850-6018-45c8-b3b8-1409129ce650 |
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| Enregistrement créé | 2010-05-21 |
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| Enregistrement modifié | 2020-04-16 |
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