| Téléchargement | - Voir le manuscrit accepté : Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans (PDF, 1002 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1016/j.abb.2009.11.027 |
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| Auteur | Rechercher : Kim, Yong-Hak; Rechercher : Lee, Cheolju; Rechercher : Go, Hayoung; Rechercher : Konishi, Kyoko1; Rechercher : Lee, Kangseok; Rechercher : Lau, Peter C. K.1; Rechercher : Yu, Myeong-Hee |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | Malachite green; Leucomalachite green; Triphenylmethane dyes; Cytochrome c; Cunninghamella elegans |
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| Résumé | We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was determined by optical absorbance spectroscopy with an extinction coefficient (E₅₅₀–₅₃₅) of 19.7 ± 6.3 mM-¹ cm-¹ and reduction potential of + 261 mV. When purified CeCyt was added into the mitochondria, the specific activity of CeCyt reached 440 ± 122 μmol min-¹ mg-¹ protein. The inhibition of MG reduction by stigmatellin, but not by antimycin A, indicated a possible linkage of CeCyt activity to the Qo site of the bc1 complex. The RT-PCR results showed tight regulation of the cecyt gene expression by reactive oxygen species. We suggest that CeCyt acts as a protein reductant for MG under oxidative stress in a stationary or secondary growth stage of this fungus. |
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| Date de publication | 2010-02-15 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 50008 |
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| Numéro NPARC | 14262109 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 6496d10b-f81e-44bd-b0b5-53afd32c457a |
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| Enregistrement créé | 2010-06-02 |
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| Enregistrement modifié | 2020-04-17 |
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