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Different iron-chelating properties of pyochelin diastereoisomers revealed by LC/MS

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/s00216-006-0443-0
AuteurRechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
FormatTexte, Article
Sujetpyochelin; pseudomonas siderophore; iron complex; electrospray ionization; liquid chromatography/mass spectrometry
Résumé
Pyochelin is a siderophore and virulence factor common to Burkholderia cepacia and several Pseudomonas strains. It is isolated from bacterial media as a mixture of two epimers, which readily equilibrate in most solvents. Experiments based on high-performance liquid chromatography/electrospray ionization mass spectrometry are reported here, allowing the investigation of the different Fe(III)-chelating properties of pyochelin diastereomers in solution without the need for labourious isolation. It is demonstrated in this study that only one of the two pyochelin diastereomers is able to chelate Fe(III); no Fe(III) complexes of the other diastereomer could be detected. The Fe(III)-pyochelin complex exhibited a 1:1 metal-to-siderophore ratio and no evidence for other stoichiometries was found.
Date de publication
Conditions d’utilisation
  • Copyright © 2007, Blackwell Publishing Ltd.
Dans
  • Analytical and Bioanalytical Chemistry 385, nº 3 (6 mai 2006) : 606–611.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 55867
1527
Numéro NPARC3538405
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Identificateur de l’enregistrement6568105e-258d-4fcf-bc00-029e7464c65f
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-22
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