| DOI | Trouver le DOI : https://doi.org/10.1093/glycob/cwaa069 |
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| Auteur | Rechercher : St Michael, Frank1; Rechercher : Cairns, Chantelle M.1; Rechercher : Fleming, Perry1; Rechercher : Vinogradov, Evgeny V.1; Rechercher : Boyce, John D.; Rechercher : Harper, Marina; Rechercher : Cox, Andrew D.1Identifiant ORCID : https://orcid.org/0000-0002-8397-9271 |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Sujet | capsular polysaccharides; Pasteurella multocida; NMR; serotyping; structure |
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| Résumé | We describe the structural characterization of the capsular polysaccharides (CPSs) of Pasteurella multocida serotypes B and E. CPS was isolated following organic solvent precipitation of the supernatant from flask grown cells. Structural analysis utilizing nuclear magnetic resonance spectroscopy enabled the determination of the CPS structures and revealed significant structural similarities between the two serotypes, but also provided an explanation for the serological distinction. This observation was extended by the development of polyclonal sera to the glycoconjugate of serotype B CPS that corroborated the structural likenesses and differences. Finally, identification of these structures enabled a more comprehensive interrogation of the genetic loci and prediction of roles for some of the encoded proteins in repeat unit biosynthesis. |
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| Date de publication | 2020-07-28 |
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| Maison d’édition | Oxford University Press |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 6bf31659-9830-497c-8c46-106881af04f9 |
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| Enregistrement créé | 2023-05-02 |
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| Enregistrement modifié | 2023-05-02 |
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