| Téléchargement | - Voir la version finale : In situ microcosms deployed at the coast of British Columbia (Canada) to study dilbit weathering and associated microbial communities under marine conditions (PDF, 1.8 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1093/femsec/fiab082 |
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| Auteur | Rechercher : Schreiber, Lars1Identifiant ORCID : https://orcid.org/0000-0003-3411-0083; Rechercher : Fortin, Nathalie1; Rechercher : Tremblay, Julien1; Rechercher : Wasserscheid, Jessica1; Rechercher : Sanschagrin, Sylvie1; Rechercher : Mason, Jennifer; Rechercher : Wright, Cynthia A; Rechercher : Spear, David; Rechercher : Johannessen, Sophia C; Rechercher : Robinson, Brian; Rechercher : King, Thomas; Rechercher : Lee, Kenneth; Rechercher : Greer, Charles W1 |
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| Affiliation | - Conseil national de recherches du Canada. Énergie, les mines et l'environnement
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| Format | Texte, Article |
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| Description physique | 16 p. |
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| Sujet | dilbit; oil biodegradation; Douglas Channel; Hecate Strait; in situ microcosms |
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| Résumé | Douglas Channel and the adjacent Hecate Strait (British Columbia, Canada) are part of a proposed route to ship diluted bitumen (dilbit). This study presents how two types of dilbit naturally degrade in this environment by using an in situ microcosm design based on dilbit-coated beads. We show that dilbit-associated n-alkanes were microbially biodegraded with estimated half-lives of 57–69 days. n-Alkanes appeared to be primarily degraded using the aerobic alkB, ladA and CYP153 pathways. The loss of dilbit polycyclic aromatic hydrocarbons (PAHs) was slower than of n-alkanes, with half-lives of 89–439 days. A biodegradation of PAHs could not be conclusively determined, although a significant enrichment of the phnAc gene (a marker for aerobic PAH biodegradation) was observed. PAH degradation appeared to be slower in Hecate Strait than in Douglas Channel. Microcosm-associated microbial communities were shaped by the presence of dilbit, deployment location and incubation time but not by dilbit type. Metagenome-assembled genomes of putative dilbit-degraders were obtained and could be divided into populations of early, late and continuous degraders. The majority of the identified MAGs could be assigned to the orders Flavobacteriales, Methylococcales, Pseudomonadales and Rhodobacterales. A high proportion of the MAGs represent currently unknown lineages or lineages with currently no cultured representative. |
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| Date de publication | 2021-06-14 |
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| Maison d’édition | Oxford |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 6c14ec8f-dcd6-4f07-855e-112166a6b353 |
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| Enregistrement créé | 2022-10-17 |
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| Enregistrement modifié | 2022-10-17 |
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