| Téléchargement | - Voir la version finale : Purification and characterization of trehalase inhibitor from hemolymph of the American cockroach, Periplaneta americana (PDF, 2.4 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1016/S0021-9258(18)71602-5 |
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| Auteur | Rechercher : Hayakawa, Yoichi; Rechercher : Jahagirdar, Ashok P.; Rechercher : Yaguchi, Makoto1; Rechercher : Downer, Roger G. H. |
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| Affiliation | - Conseil national de recherches du Canada
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| Format | Texte, Article |
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| Résumé | An endogenous proteinaceous inhibitor of trehalase (alpha,alpha-trehalose-1-glucohydrolase: EC 3.2.1.28) has been isolated and purified from the serum of resting adult American cockroaches, Periplaneta americana. Purification procedures involved decreasing ionic strength, gel filtration, and reversed phase high performance liquid chromatography. Homogeneity was confirmed by polyacrylamide gel electrophoresis and end group analysis. The purified protein inhibited trehalase activity in a dose-dependent manner and was estimated to have a molecular weight of 86,000 and to contain sugar chains. An automated gas-phase sequencer was used to determine the following sequence for the N-terminal amino acid residues: H-Ala-Ilu-Pro-Thr-Pro-His-Val-Tyr-Lys-Val-X-Val-Pro-Asp-Gly-Ala-Le u-Asn-Asp. |
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| Date de publication | 1989-09-25 |
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| Maison d’édition | American Society for Biochemistry and Molecular Biology |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | HAYAKAWA1989 |
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| Numéro NPARC | 9364137 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 73d24ca6-5b46-40a4-8b61-9f75a5a37b1f |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2021-09-22 |
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