Internal ribosome-binding site directs expression of parathyroid hormone analogue (8-84) in Escherichia coli

DOI	Trouver le DOI : https://doi.org/10.1016/S0006-291X(05)81444-5
Auteur	Rechercher : Sung, Wing; Rechercher : Luk, C.; Rechercher : Zahab, D.; Rechercher : Barbier, J.; Rechercher : Lafontaine, M.; Rechercher : Willick, Gordon
Format	Texte, Article
Résumé	Expression of the human parathyroid hormone (PTH) gene in E. coli yielded intact PTH and PTH-(8–84). To determine if PTH-(8–84) is the result of a competing translation initiated from methionine codon-8 or degradation of the intact PTH, twelve new gene constructs with or without an internal ribosome-binding site (iRBS) in the PTH-(1–5) region were prepared via substitution with degenerate codons. Expression of constructs without iRBS produced only intact PTH. Constructs with weak iRBS, including one that resembles the cDNA sequence, yielded PTH-(8–84) as a minor product. In contrast, constructs with strong iRBS produced predominantly or exclusively this shorter analogue.
Date de publication	1991-11-27
Maison d’édition	Elsevier
Dans	Biochemical and Biophysical Research Communications 181, nº 1 : 481–485.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	SUNG1991A
Numéro NPARC	9375355
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	7489d13c-f74f-4710-b60e-3e7ca52ec061
Enregistrement créé	2009-07-10
Enregistrement modifié	2020-03-17