Two-stage cell shrinkage and the OER for radiation-induced apoptosis of rat thymocytes

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1080/09553009314551791
Auteur	Rechercher : Klassen, N. V.¹; Rechercher : Walker, P. R.²; Rechercher : Ross, C. K.¹; Rechercher : Cygler, J.; Rechercher : Lach, B.
Affiliation	Conseil national de recherches du Canada. Institut des étalons nationaux de mesure du CNRC Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
Format	Texte, Article
Résumé	Suspensions of rat thymocytes were given 0·09—100 Gy using 60Co γ-rays. The radiation-induced changes in the thymocytes were examined from minutes to hours postirradiation using electron microscopy, agarose gel electrophoresis, staining and Coulter Counter sizing. Sizing by Coulter Counter showed, for the first time, that thymocytes which undergo apoptosis shrink in two distinct stages, first by a sudden decrease from an original volume of 99 µm3 to a volume of 76 µm3, followed by a gradual decrease to 57 µm3 over the space of a few hours. The oxygen enhancement ratio for apoptosis was measured to be about 3·5, similar to the value for reproductive death for many mammalian cells.
Date de publication	1993
Dans	International Journal of Radiation Biology 64, nº November 5 (1993) : 571–581.
Langue	anglais
Numéro du CNRC	482 KLASSEN1993
Numéro NPARC	8900062
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	7538b5e1-90e9-4e85-8791-195683895b77
Enregistrement créé	2009-04-22
Enregistrement modifié	2020-04-24

Date de modification :: 2024-10-01