| DOI | Trouver le DOI : https://doi.org/10.1016/S0044-328X(79)80208-1 |
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| Auteur | Rechercher : Brar, D.S.1; Rechercher : Rambold, S.1; Rechercher : Gamborg, O.1; Rechercher : Constabel, F.1 |
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| Affiliation | - Conseil national de recherches du Canada. Laboratoire régional des prairies
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| Format | Texte, Article |
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| Sujet | Sorghum bicolor; Zea mays; cell suspension culture; plant regeneration; polyploidization |
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| Résumé | A fast growing cell suspension culture of sorghum (Sorghum bicolor L. Moench) has been established. Out of 15 genetic strains, cv. GPR-168 responded most favorably to conditions for callus formation. The callus was friable and upon transfer to liquid medium produced cell suspension cultures. During 96 h of subculturing the cells showed mitotic indices of 1.0 to 6.5 %. Plant regeneration from callus derived from seedlings and immature embryos was achieved in two sorghum strains. Cytology of callus, cell suspension cultures, and regenerated plants indicated a strong prevalence of polyploidization.
The study also includes observations on a fast growing cell culture of corn. The culture had a doubling time of 48 h and during a 72 h period showed mitotic indices from 3.6 to 10.9 %. Protoplasts were readily isolated from the cell culture, but spontaneous fusion occurred extensively. On average 52 % of the isolated protoplasts were multinucleated. Attempts to eliminate the phenomenon were not successful. During subsequent culture some of the protoplasts regenerated cell walls and limited division occurred. |
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| Date de publication | 1979-12 |
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| Maison d’édition | Elsevier |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | S0044328X79802081 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 75f26d25-7ed9-4a5b-b83a-5bc2c49b8cda |
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| Enregistrement créé | 2023-07-25 |
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| Enregistrement modifié | 2023-07-25 |
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