| Téléchargement | - Voir le manuscrit accepté : Rapid identification of triacylglycerol-estolides in plant and fungal oils (PDF, 1.9 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1016/j.indcrop.2011.10.026 |
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| Auteur | Rechercher : Zhang, Haixia1; Rechercher : Olson, Douglas J. H.1; Rechercher : Van, Dang1; Rechercher : Purves, Randy W.1; Rechercher : Smith, Mark A.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
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| Format | Texte, Article |
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| Sujet | estolide; Lesquerella fendleri; Lesquerella lyrata; ergot (Claviceps purpurea); seed oil; hydroxy fatty acids |
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| Résumé | Triacylglycerol-estolides are components of the storage oil of certain plant and fungal species and are generally associated with the presence of fatty acids containing hydroxyl groups. These unusual acyl-glycerols can easily go undetected when oils are analysed by techniques such as gas chromatography of fatty acid methyl esters, or thin layer chromatography. We describe the detection of TAG-estolides in intact seeds of Lesquerella lyrata and whole sclerotia of the ergot fungus Claviceps purpurea using 1H MAS-NMR for non destructive analysis. We also conducted analysis of small amounts of oil by MALDI-TOF MS to clearly show the presence of TAG-estolides and to rapidly characterize their acyl composition. The matrix used in this work was 2,4,6-trihydroxyacetophenone (THAP) made up in sodium chloride-saturated solvent. We were able to confirm the presence of TAG-estolides with no free hydroxyl groups in the fungal oil, and TAG-estolides with free hydroxyl groups in the oil of Lesquerella lyrata. The development of a technique for the rapid identification of TAG-estolides in oil samples will simplify the detection of these novel lipids in plant and fungal species. |
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| Date de publication | 2012-01-10 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 20193 |
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| Numéro NPARC | 19547514 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 7851e344-6ee8-4c3a-ad27-de43a7edf577 |
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| Enregistrement créé | 2012-04-17 |
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| Enregistrement modifié | 2020-04-21 |
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