| DOI | Trouver le DOI : https://doi.org/10.4049/jimmunol.1200639 |
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| Auteur | Rechercher : Tzelepis, F.1; Rechercher : Joseph, J.1; Rechercher : Haddad, E.K.1; Rechercher : MacLean, S.1; Rechercher : Dudani, R.; Rechercher : Agenes, F.; Rechercher : Peng, S.L.; Rechercher : Sekaly, R.-P.; Rechercher : Sad, S.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | actin; BIM protein; cytokine; lysosome associated membrane protein 1; protein Bid; PUMA protein; RAG1 protein; transcription factor FKHRL1; animal cell; animal experiment; animal model; antigen presentation; apoptosis; CD8+ T lymphocyte; cell expansion; cell survival; cytokine release; degranulation; effector cell; female; listeriosis; lymphocyte function; memory T lymphocyte; mouse; pre T lymphocyte; protein analysis; protein deficiency; protein expression; protein function; wild type; Antigen Presentation; Antigens, Bacterial; Apoptosis; Apoptosis Regulatory Proteins; CD8-Positive T-Lymphocytes; Cytokines; Cytotoxicity, Immunologic; Female; Forkhead Transcription Factors; Homeodomain Proteins; Immunologic Memory; L-Selectin; Listeria monocytogenes; Listeriosis; Lymphocyte Activation; Lymphocyte Subsets; Lymphokines; Lysosome-Associated Membrane Glycoproteins; Membrane Proteins; Mice; Mice, Inbred C57BL; Ovalbumin; Proto-Oncogene Proteins; Receptors, Interleukin-7; Tumor Suppressor Proteins |
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| Résumé | CD8+ T cells undergo rapid expansion during infection with intracellular pathogens, which is followed by swift and massive culling of primed CD8+ T cells. The mechanisms that govern the massive contraction and maintenance of primed CD8+ T cells are not clear. We show in this study that the transcription factor, FoxO3a, does not influence Ag presentation and the consequent expansion of CD8+ T cell response during Listeria monocytogenes infection, but plays a key role in the maintenance of memory CD8+ T cells. The effector function of primed CD8+ T cells as revealed by cytokine secretion and CD107a degranulation was not influenced by inactivation of FoxO3a. Interestingly, FoxO3a-deficient CD8+ T cells displayed reduced expression of proapoptotic molecules BIM and PUMA during the various phases of response, and underwent reduced apoptosis in comparison with wild-type cells. A higher number of memory precursor effector cells and memory subsets was detectable in FoxO3a-deficient mice compared with wild-type mice. Furthermore, FoxO3a-deficient memory CD8+ T cells upon transfer into normal or RAG1-deficient mice displayed enhanced survival. These results suggest that FoxO3a acts in a cell-intrinsic manner to regulate the survival of primed CD8+ T cells. Copyright © 2013 by The American Association of Immunologists, Inc. |
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| Date de publication | 2013 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21269840 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 7abb551f-c5ff-42ba-8b21-d9f8088bf917 |
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| Enregistrement créé | 2013-12-13 |
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| Enregistrement modifié | 2020-04-22 |
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