| Téléchargement | - Voir la version finale : Differentiation of adipose-derived stem cells into vascular smooth muscle cells for tissue engineering applications (PDF, 3.2 Mio)
- Voir les données supplémentaires : Differentiation of adipose-derived stem cells into vascular smooth muscle cells for tissue engineering applications (PDF, 197 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.3390/biomedicines9070797 |
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| Auteur | Rechercher : Yogi, Alvaro1; Rechercher : Rukhlova, Marina1; Rechercher : Charlebois, Claudie1; Rechercher : Tian, Ganghong2; Rechercher : Stanimirovic, Danica B.1; Rechercher : Moreno, Maria J.1 |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
- Conseil national de recherches du Canada. Dispositifs médicaux
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| Format | Texte, Article |
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| Sujet | adipose stem cells; tissue engineering; differentiation; vascular smooth muscle; contraction |
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| Résumé | Synthetic grafts have been developed for vascular bypass surgery, however, the risks of thrombosis and neointimal hyperplasia still limit their use. Tissue engineering with the use of adipose-derived stem cells (ASCs) has shown promise in addressing these limitations. Here we further characterized and optimized the ASC differentiation into smooth muscle cells (VSMCs) induced by TGF-β and BMP-4. TGF-β and BMP-4 induced a time-dependent expression of SMC markers in ASC. Shortening the differentiation period from 7 to 4 days did not impair the functional property of contraction in these cells. Stability of the process was demonstrated by switching cells to regular growth media for up to 14 days. The role of IGFBP7, a downstream effector of TGF-β, was also examined. Finally, topographic and surface patterning of a substrate is recognized as a powerful tool for regulating cell differentiation. Here we provide evidence that a non-woven PET structure does not affect the differentiation of ASC. Taken together, our results indicate that VSMCs differentiated from ASCs are a suitable candidate to populate a PET-based vascular scaffolds. By employing an autologous source of cells we provide a novel alternative to address major issues that reduces long-term patency of currently vascular grafts. |
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| Date de publication | 2021-07-09 |
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| Maison d’édition | MDPI |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC-HHT-53527A |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 7da46db0-33b6-4796-be28-78de9e3e4197 |
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| Enregistrement créé | 2021-11-08 |
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| Enregistrement modifié | 2021-11-08 |
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