| Téléchargement | - Voir le manuscrit accepté : Design and fabrication of 3D porous scaffolds to facilitate cell-based gene therapy (PDF, 1.6 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1089/ten.tea.2006.0418 |
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| Auteur | Rechercher : El-Ayoubi, Rouwayda1; Rechercher : Eliopoulos, Nicoletta; Rechercher : DiRaddo, Robert1; Rechercher : Galipeau, Jacques; Rechercher : Yousefi, Azizeh-Mitra1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des matériaux industriels du CNRC
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| Format | Texte, Article |
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| Sujet | 3D scaffold; solid free-form fabrication; porogen leaching technique; gene therapy; erythropoietin; mesenchymal stromal cells |
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| Résumé | Biomaterials capable of efficient gene delivery by embedded cells provide a fundamental tool for the treatment of acquired or hereditary diseases. A major obstacle is maintaining adequate nutrient and oxygen diffusion to cells within the biomaterial. In this study, we combined the solid free-form fabrication and porogen leaching techniques to fabricate three-dimensional scaffolds, with bimodal pore size distribution, for cell-based gene delivery. The objective of this study was to design micro-/macroporous scaffolds to improve cell viability and drug delivery. Murine bone marrow-derived mesenchymal stromal cells (MSCs) genetically engineered to secrete erythropoietin (EPO) were seeded onto poly-L-lactide (PLLA) scaffolds with different microporosities. Over a period of 2 weeks in culture, an increase in cell proliferation and metabolic activity was observed with increasing scaffold microporosity. The concentration of EPO detected in supernatants also increased with increasing microporosity level.Our study shows that these constructs can promote cell viability and release of therapeutic proteins, and clearly demonstrates their capacity for a dual role as scaffolds for tissue regeneration and as delivery systems for soluble gene products. |
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| Date de publication | 2007-01-01 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 48944 |
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| Numéro NPARC | 15774997 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 864be268-391b-4060-a174-26cc36be6bf1 |
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| Enregistrement créé | 2010-07-06 |
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| Enregistrement modifié | 2020-05-10 |
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