| DOI | Trouver le DOI : https://doi.org/10.1016/j.carres.2013.03.011 |
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| Auteur | Rechercher : Vitiazeva, V.; Rechercher : Li, J.1; Rechercher : Hood, D.W.; Rechercher : Richard Moxon, E.; Rechercher : Schweda, E.K.H. |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | D-glycero-D-manno-Heptose; Haemophilus influenzae; Lipopolysaccharides; LosA1; Sialyllacto-N-neotetraose; Capillary electrophoresis; Electrospray ionization; Nuclear magnetic resonance spectroscopy; Mass spectrometry; heptose; lipopolysaccharide; oligosaccharide; sialic acid; tetrasaccharide; bacterial strain; capillary electrophoresis; carbohydrate analysis; deacylation; dephosphorylation; electrospray mass spectrometry; Haemophilus influenzae; high performance liquid chromatography; hydrolysis; methylation; nuclear magnetic resonance spectroscopy; sequence analysis; sialylation; tandem mass spectrometry; Haemophilus influenzae |
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| Résumé | We report the novel branching pattern in lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strain 1232. The strain expressed the β-D-Glcp-(1→4)-[α-D-Galp-(1→4)-β-D-Galp- (1→7)]-D-α-D-Hepp-(1→6)-β-D-Glcp chain linked to the proximal heptose (HepI) of the conserved triheptosyl inner-core moiety of NTHi LPS: L-α-D-HepIIIp-(1→2)-[PEtn→6]-L-α-D-HepIIp-(1→3)- L-α-DHepIp-( 1→5)-[PPEtn→4]-α-Kdop-(2→6)-lipid A. The structure has been elucidated using NMR spectroscopy, electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis coupled to electrospray ionization tandem mass spectrometry (CE-ESI-MSn) on O-deacylated LPS and core oligosaccharide (OS) materials, as well as HPLC-ESI-MSn on permethylated, dephosphorylated OS. It was also found that a tetrasaccharide unit bearing sialic acid [α-Neu5Ac-(2→3)-β-D-Galp-(1→4)- β-D-GlcNAcp-(1→3)-β-D-Galp-(1→] could substitute O-4 of the β-D-Glcp linked to HepI. In addition, the distal heptose (HepIII) was substituted by PCho→6-β-D-Galp-(1→ at the O-2 position. © 2013 Published by Elsevier Ltd. |
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| Date de publication | 2013 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21269713 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 865e9dc6-3703-427e-b742-a099cd4a40e6 |
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| Enregistrement créé | 2013-12-13 |
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| Enregistrement modifié | 2020-04-22 |
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