| DOI | Trouver le DOI : https://doi.org/10.1016/j.ymthe.2005.07.058 |
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| Auteur | Rechercher : Durocher, Yves1; Rechercher : Pham, Phuong Lan1; Rechercher : St-Laurent, Gilles1; Rechercher : Jacob, Danielle1; Rechercher : Cass, Brian1; Rechercher : Nalbantoglu, Josephine; Rechercher : Kamen, Amine1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Résumé | Recombinant adeno-associated viruses (rAAV) represent a promising gene therapy vector that may be used in the treatment of diverse human diseases. The major obstacle to broaden their usage is the availability of a production process able to provide sufficient quantities for preclinical and human trials. We present here a successful process for rAAV-2 production in low-serum and serum-free medium performed in a 3L stirred-tank bioreactor. The process is based on the triple transfection of suspension-growing HEK293 cells employing polyethylenimine (PEI) as the DNA carrier. Production of AAV-GFP in a 3L serum-free medium bioreactor yielded titers of 5.1 x 1011 infectious viral particles (IVP), corresponding to 2 x 1012 viral genome (VG) or 6.8 x 1012 viral particles (VP). The cell-specific and total viral titers obtained in suspension culture were about three-fold higher to those obtained with adherent cells. The process is very simple and robust as it does not require a medium exchange, either before or after transfection, making it particularly attractive for the generation of large and homogeneous stocks of rAAV vectors. |
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| Date de publication | 2005-05 |
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| Maison d’édition | Elsevier |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23001981 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 8786f6cb-d0bd-42a4-b52c-3a4ac0e9808f |
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| Enregistrement créé | 2017-07-12 |
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| Enregistrement modifié | 2020-04-07 |
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