DOI | Trouver le DOI : https://doi.org/10.1016/j.bioorg.2006.12.004 |
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Auteur | Rechercher : Noestheden, Matthew1; Rechercher : Hu, Qingyan1; Rechercher : Tay, Li-Lin2; Rechercher : Tonary, Angela M.1; Rechercher : Stolow, Albert1; Rechercher : MacKenzie, Roger3; Rechercher : Tanha, Jamshid3; Rechercher : Pezacki, John Paul1 |
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Affiliation | - Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
- Conseil national de recherches du Canada. Institut des sciences des microstructures du CNRC
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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Format | Texte, Article |
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Sujet | Antigen-Antibody Reactions; Benzoic Acids; Molecular Structure; Protein Structure, Tertiary; Sensitivity and Specificity; Serum Albumin, Bovine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrum Analysis, Raman; Staphylococcal Protein A; Staphylococcus aureus; Structure-Activity Relationship; Succinimides; Surface Plasmon Resonance; Vibration |
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Résumé | A recombinant VH single-domain antibody recognizing staphylococcal protein A was functionalized on reactive lysine residues with N-hydroxysuccimidyl-activated 4-cyanobenzoate. Surface plasmon resonance analysis of antibody-antigen binding revealed that modified and unmodified antibodies bound protein A with similar affinities. Raman imaging of the modified antibodies indicated that the benzonitrile group provides vibrational contrast enhancement in a region of the electromagnetic spectrum that is transparent to cellular materials. Thus, the modified single-domain antibody may be amenable to detecting protein A from samples of the human pathogen Staphylococcus aureus using vibronic detection schemes such as Raman and coherent anti-Stokes Raman scattering. The generality of this labeling strategy should make it applicable to modifying an array of proteins with varied structure and function. |
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Date de publication | 2007-06 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 12327431 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | 87e5f5e7-ef0c-4969-a865-c6bbb6b2c6fc |
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Enregistrement créé | 2009-09-10 |
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Enregistrement modifié | 2020-05-10 |
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