| DOI | Trouver le DOI : https://doi.org/10.1021/acs.jafc.0c02024 |
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| Auteur | Rechercher : Miller, Todd R.Identifiant ORCID : https://orcid.org/0000-0002-2113-1662; Rechercher : Xiong, Ame; Rechercher : Deeds, Jonathan R.; Rechercher : Stutts, Whitney L.; Rechercher : Samdal, Ingunn A.Identifiant ORCID : https://orcid.org/0000-0001-5225-2494; Rechercher : Løvberg, Kjersti E.; Rechercher : Miles, Christopher O.1 |
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| Affiliation | - Conseil national de recherches du Canada. Centre de recherche en métrologie
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| Format | Texte, Article |
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| Sujet | microcystin; Microcystis; harmful algae; Aphanizomenon flos-aquae; ELISA; food supplement; LC-MS; PPIA |
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| Résumé | Microcystins (MCs) are hepatotoxic heptapeptides produced by cyanobacteria and are potent inhibitors of protein phosphatases in eukaryotic cells. Algae for dietary supplements are harvested from outdoor environments and can be contaminated with MCs. Monitoring of MCs in these products is necessary but is complicated by their structural diversity (>250 congeners). We used a combination of protein phosphatase inhibition assay (PPIA), ELISA, LC−MS/MS, and nontargeted LC–high-resolution MS (LC–HRMS) with thiol derivatization to characterize the total MCs in 18 algal dietary supplements. LC–MS/MS revealed that some products contained >40 times the maximum acceptable concentration (MAC) of 1 μg/g MCs, but ELISA and PPIA showed up to 50–60 times the MAC. LC–HRMS identified all congeners targeted by LC–MS/MS plus MC-(H4)YR contributing up to 18% of total MCs, along with numerous minor MCs. Recommended dosages of the products greater than the MAC would result in 2.6–75 times the tolerable daily intake, presenting a risk to consumers. This study confirms the need for monitoring these products and presents strategies to fully describe the total MC pool in environmental samples and algal products. |
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| Date de publication | 2020-06-29 |
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| Maison d’édition | American Chemical Society |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 8da22064-cce5-4baf-8a06-55160947ff88 |
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| Enregistrement créé | 2020-09-01 |
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| Enregistrement modifié | 2020-10-30 |
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