Optimum levels of sulphate and iron for the cultivation of pure cultures of methanogens in synthetic media

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1111/j.1365-2672.1978.tb04235.x
Auteur	Rechercher : Patel, G.¹; Rechercher : Khan, A.¹; Rechercher : Roth, L.²
Affiliation	Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC Conseil national de recherches du Canada
Format	Texte, Article
Résumé	Experiments on the effects, of sulphate and iron content of medium on the growth of Methanobacterium strain M.O.H., Methanospirllum hungatii GPI, and an unidentified strain similar in characteristics to Methanobacterium formicicum, indicated that the 4.0 mM of sulphate normally incorporated into laboratory media was above the optimum level, while the iron content normally incorporated (0.01–0.08 mM) was below the optimum requirement of these methanogens. The tests were made in synthetic media containing acetate, carbon dioxide and hydrogen as carbon and energy sources, and a cysteine-sodium sulphide solution as a reducing agent. The optimum sulphate requirement for Methanobacterium strain M.O.H., M. hungatii, and the unidentified methanogen was in the range of 0.16–0.52 mM. An absence of sulphate in the medium decreased significantly (95% confidence level) the growth of these cultures. The optimum requirement of Fe++ was in the range of 0.3–0.9 mM. Addition of Fe+++ greater than 0.10 mM to pre-reduced medium, or the addition of greater than 0.15 mM during preparation of medium resulted in precipitation of iron. A method for the preparation of medium without formation of precipitate is described and its contribution to improving growth and methanogenesis discussed.
Date de publication	1978
Dans	Journal of Applied Microbiology 45, nº 3 (1978) : 347–356.
Langue	anglais
Numéro du CNRC	PATEL1978 NRCC 16602
Numéro NPARC	9388167
Exporter la notice	Exporter en format RIS
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Identificateur de l’enregistrement	92f371e7-cd92-4eea-8912-121c78789a5b
Enregistrement créé	2009-07-10
Enregistrement modifié	2020-03-13

Date de modification :: 2024-07-06