Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1016/j.fgb.2014.05.006
Auteur	Rechercher : Kolbusz, Magdalena Anna; Rechercher : Di Falco, Marcos; Rechercher : Ishmael, Nadeeza; Rechercher : Marqueteau, Sandrine; Rechercher : Moisan, Marie-Claude; Rechercher : Baptista, Cassio da Silva; Rechercher : Powlowski, Justin; Rechercher : Tsang, Adrian
Format	Texte, Article
Sujet	Myceliophthora thermophila; Biomass degradation; Carbohydrate-active enzymes; RNA-Seq; Mass spectrometry
Résumé	Myceliophthora thermophila is a thermophilic fungus whose genome encodes a wide range of carbohydrate-active enzymes (CAZymes) involved in plant biomass degradation. Such enzymes have potential applications in turning different kinds of lignocellulosic feedstock into sugar precursors for biofuels and chemicals. The present study examined and compared the transcriptomes and exoproteomes of M. thermophila during cultivation on different types of complex biomass to gain insight into how its secreted enzymatic machinery varies with different sources of lignocellulose. In the transcriptome analysis three monocot (barley, oat, triticale) and three dicot (alfalfa, canola, flax) plants were used whereas in the proteome analysis additional substrates, i.e. wood and corn stover pulps, were included. A core set of 59 genes encoding CAZymes was up-regulated in response to both monocot and dicot straws, including nine polysaccharide monooxygenases and GH10, but not GH11, xylanases. Genes encoding additional xylanolytic enzymes were up-regulated during growth on monocot straws, while genes encoding additional pectinolytic enzymes were up-regulated in response to dicot biomass. Exoproteome analysis was generally consistent with the conclusions drawn from transcriptome analysis, but additional CAZymes that accumulated to high levels were identified. Despite the wide variety of biomass sources tested some CAZy family members were not expressed under any condition. The results of this study provide a comprehensive view from both transcriptome and exoproteome levels, of how M. thermophila responds to a wide range of biomass sources using its genomic resources.
Date de publication	2014-05-29
Dans	Fungal Genetics and Biology 72 (29 mai 2014) : 10–20.
Langue	anglais
Publications évaluées par des pairs	Oui
Publication du CNRC	Cette publication n’est pas du CNRC Ce sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC.
Numéro NPARC	21272895
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	95f31503-b475-41c5-8c80-2c6bf9e545c1
Enregistrement créé	2014-12-03
Enregistrement modifié	2020-04-22

Date de modification :: 2024-07-19