| DOI | Trouver le DOI : https://doi.org/10.1002/pmic.200900447 |
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| Auteur | Rechercher : Costain, Willard J.1; Rechercher : Haqqani, Arsalan S.1; Rechercher : Rasquinha, Ingrid1; Rechercher : Giguere, Marie-Soleil1; Rechercher : Slinn, Jacqueline1; Rechercher : Zurakowski, Bogdan1; Rechercher : Stanimirovic, Danica B.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | Animal proteomics; Cerebral ischemia; Hippocampus; Isotope-coded affinity tags; Prosaposin; Synaptosome |
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| Résumé | Cerebral ischemia (CI) induces dramatic changes in synaptic structure and function that precedes delayed post-ischemic neuronal death. Here, a proteomic analysis was used to identify the effects of focal CI on synaptosomal protein levels. Contralateral and ipsilateral synaptosomes, prepared from adult mice subjected to 60 min middle cerebral artery occlusion, were isolated following 3, 6 and 20 h of reperfusion. Synaptosomal protein samples (n=3) were labeled using the cleavable ICATTM system prior to analysis with nanoLC-MS/MS. Each sample was analyzed by LC-MS to identify differential expressions using InDEPT software and differentially expressed peptides were identified by targeted LC-MS/MS. A total of 62 differentially expressed proteins were identified and Gene Ontology classification (cellular component) indicated that the majority of the proteins were located in the mitochondria and other components consistent with synaptic localization. The observed alterations in synaptic protein levels poorly correlated with gene expression, indicating the involvement of post-transcriptional regulatory mechanisms in determining post-ischemic synaptic protein content. Additionally, immunohistochemistry analysis of prosaposin (Psap) and saposin C (SapC) indicates that CI disrupts Psap processing and glycosphingolipid metabolism. These results demonstrate that the synapse is adversely affected by CI and may play a role in mediating post-ischemic neuronal viability. |
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| Date de publication | 2010-09-01 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 17480985 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 98110c9d-04e1-4add-9043-d38800db88f7 |
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| Enregistrement créé | 2011-03-30 |
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| Enregistrement modifié | 2020-04-17 |
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