Process development for production of recombinant human insulin-like growth factor-I in Escherichia coli

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1038/sj.jim.2900773
Auteur	Rechercher : Chung, B. H.; Rechercher : Choi, Y. J.; Rechercher : Yoon, S. H.; Rechercher : Lee, S. Y.; Rechercher : Lee, Y. I.
Format	Texte, Article
Résumé	Fed-batch cultures were carried out to overproduce human insulin-like growth factor I (IGF-I) in Escherichia coli. The effects of carbon sources (glucose or glycerol) and induction time on cell growth and IGF-I production were investigated in more detail. Glycerol was a better carbon source than glucose for IGF-I production in fed-batch culture. Induction at the mid-exponential phase with glycerol as a carbon source in the pH-stat fed-batch culture was optimal for IGF-I production. Under this condition, 2.8 g L[SUP-1] of fusion IGF-I was produced as inclusion bodies. We have also developed downstream processing for preparative scale purification of IGF-I from the fusion protein produced by the fed-batch culture using glycerol as a carbon source. After the fusion protein expressed was solubilized in 8 M urea and cleaved with hydroxylamine, the released IGF-I was purified by cation exchange chromatography, refolding and preparative scale reverse phase HPLC (rp-HPLC) to give recombinant IGF-I of >98% purity. The biological activities of the purified IGF-I were measured and found to be identical to those of commercial IGF-I.
Date de publication	2000-02-01
Dans	Journal of Industrial Microbiology and Biotechnology 24, nº 2 : 94–99.
Langue	anglais
Publications évaluées par des pairs	Oui
Publication du CNRC	Cette publication n’est pas du CNRC Ce sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC.
Numéro NPARC	23001828
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Identificateur de l’enregistrement	983f89c3-6b7b-46b7-a7f0-6556540cf123
Enregistrement créé	2017-04-13
Enregistrement modifié	2020-03-26

Date de modification :: 2024-04-19