Nutrient utilization during bioreactor culture, and maturation of somatic embryo cultures of Picea mariana and Picea glauca-engelmannii

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1007/BF02632121
Auteur	Rechercher : Tautorus, T. E.¹; Rechercher : Lulsdorf, M. M.¹; Rechercher : Kikcio, S. I.¹; Rechercher : Dunstan, D. I.¹
Affiliation	Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
Format	Texte, Article
Sujet	spruce; somatic embryo; shake-flask; bioreactor; maturation
Résumé	Embryogenic cell cultures ofPicea mariana (black spruce) and the species complexPicea glauca-engelmannii (interior spruce) were maintained either as suspensions in liquid medium in 250 or 500-ml-capacity shake-flasks, 7-liter-capacity airlift or mechanically stirred bioreactors, or on agar-solidified medium. Cultures from each of the maintenance conditions were subsequently transferred to agar-solidified LP medium containing 40µM (±) -abscisic acid for maturation into cotyledonary stage embryos. For both species, the highest maturation frequency resulted from cultures grown in the airlift bioreactor. With black spruce cells grown in the airlift bioreactor containing LP medium with 60 mM sucrose, a maximum of 7.1 g·liter⁻¹ dry weight and 2892 embryos·ml−1 were obtained after 15 days. For interior spruce cells, a maximum dry weight of 5.9 g·liter⁻¹ and 2698 embryos·ml⁻¹ were obtained after 21 to 30 days. During culture over 2 wk, ammonia was almost completely utilized by both species, wherease nitrate was depleted to 40% of the initial concentration. Sucrose was rapidly hydrolyzed to glucose and fructose by both species. Black spruce cultures preferentially metabolized glucose, whereas interior spruce preferentially metabolized fructose. Improved growth of interior spruce cells in mechanically stirred bioreactors occurred when cultured in LP medium with 60 mM fructose as the sole carbon source.
Date de publication	1994-01
Maison d’édition	Springer Link
Dans	In Vitro Cellular & Developmental Biology - Plant 30, nº 1 (janvier 1994) : 58–63.
Langue	anglais
Publications évaluées par des pairs	Oui
Identificateur	BF02632121 NRCC-36479
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	9d1e3a78-ffa9-42c4-9fa4-91155238e5a6
Enregistrement créé	2023-10-25
Enregistrement modifié	2023-10-25

Date de modification :: 2024-07-06