| DOI | Trouver le DOI : https://doi.org/10.1074/jbc.M115.654798 |
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| Auteur | Rechercher : Pan, X.; Rechercher : Chen, G.; Rechercher : Kazachkov, M.1; Rechercher : Greer, M.S.; Rechercher : Caldo, K.M.P.; Rechercher : Zou, J.1; Rechercher : Weselake, R.J. |
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| Affiliation | - Conseil national de recherches Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Article |
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| Sujet | fatty acids; flax; glycerol; linen; lipids; oilseeds; phospholipids; seed; embryo |
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| Résumé | Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs to be further explored. By using in vivo and in vitro approaches, we demonstrated that the PC deacylation reaction catalyzed by the reverse action of acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) can transfer PUFAs on PC directly into the acyl-CoA pool, making these PUFAs available for the diacylglycerol acyltransferase (DGAT)-catalyzed reaction for TAG production. Two types of yeast mutants were generated for in vivo and in vitro experiments, respectively. Both mutants provide a null background with no endogenous TAG forming capacity and an extremely low LPCAT activity. In vivo experiments showed that co-expressing flax DGAT1-1 and LPCAT1 in the yeast quintuple mutant significantly increased 18-carbon PUFAs in TAG with a concomitant decrease of 18-carbon PUFAs in phospholipid. We further showed that after incubation of sn-2-[14C]acyl-PC, formation of [14C]TAG was only possible with yeast microsomes containing both LPCAT1 and DGAT1-1. Moreover, the specific activity of overall LPCAT1 and DGAT1-1 coupling process exhibited a preference for transferring 14C-labeled linoleoyl or linolenoyl than oleoyl moieties from the sn-2 position of PC to TAG. Together, our data support the hypothesis of biochemical coupling of the LPCAT1-catalyzed reverse reaction with the DGAT1-1-catalyzed reaction for incorporating PUFAs into TAG. This process represents a potential route for enriching TAG in PUFA content during seed development in flax. |
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| Date de publication | 2015 |
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| Maison d’édition | American Society for Biochemistry and Molecular Biology |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21276931 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | a255a83a-3582-401e-964b-b243f20d2a10 |
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| Enregistrement créé | 2015-11-10 |
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| Enregistrement modifié | 2020-04-22 |
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