| Téléchargement | - Voir le manuscrit accepté : Differences in lactococcal cell wall polysaccharide structure are major determining factors in bacteriophage sensitivity (PDF, 1.3 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1128/mBio.00880-14 |
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| Auteur | Rechercher : Ainsworth, Stuart; Rechercher : Sadovskaya, Irina; Rechercher : Vinogradov, Evguenii1; Rechercher : Courtin, Pascal; Rechercher : Guerardel, Yann; Rechercher : Mahony, Jennifer; Rechercher : Grard, Thierry; Rechercher : Cambillau, Christian; Rechercher : Chapot-Chartier, Marie-Pierre; Rechercher : van Sinderen, Douwe |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Sujet | bacterial polysaccharide; cell surface receptor; cell wall polysaccharide; pentasaccharide; unclassified drug; bacterial cell wall; bacterial genome; bacterial strain; bacteriophage; bacterium mutant; biochemical composition; biosynthesis; gene cluster; genetic association; genetic complementation; genetic variability; host cell; host range; Lactococcus lactis; nucleotide sequence; sensitivity analysis; structure analysis |
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| Résumé | Analysis of the genetic locus encompassing a cell wall polysaccharide (CWPS) biosynthesis operon of eight strains of Lactococcus lactis, identified as belonging to the same CWPS type C genotype, revealed the presence of a variable region among the strains examined. The results allowed the identification of five subgroups of the C type named subtypes C1 to C5. This variable region contains genes encoding glycosyltransferases that display low or no sequence homology between the subgroups. In this study, we purified an acidic polysaccharide from the cell wall of L. lactis 3107 (subtype C2) and confirmed that it is structurally different from the previously established CWPS of subtype C1 L. lactis MG1363. The CWPS of L. lactis 3107 is composed of pentasaccharide repeating units linked by phosphodiester bonds with the structure 6-α-Glc-3-β-Galf-3-β-GlcNAc-2-β-Galf-6-α-GlcNAc-1-P. Combinations of genes from the variable region of subtype C2 were introduced into a mutant of subtype C1 L. lactis NZ9000 deficient in CWPS biosynthesis. The resulting recombinant mutant synthesized a polysaccharide with a composition characteristic of that of subtype C2 L. lactis 3107 and not wild-type C1 L. lactis NZ9000. By challenging the recombinant mutant with various lactococcal phages, we demonstrated that CWPS is the host cell surface receptor of tested bacteriophages of both the P335 and 936 groups and that differences between the CWPS structures play a crucial role in determining phage host range. |
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| Date de publication | 2014-05-06 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21272859 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | a5da84fd-8636-4874-b20e-62c20cd0e685 |
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| Enregistrement créé | 2014-12-03 |
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| Enregistrement modifié | 2020-06-04 |
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