| DOI | Trouver le DOI : https://doi.org/10.1166/jnn.2014.9076 |
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| Auteur | Rechercher : Fatehi, D.1; Rechercher : Baral, T.N.1; Rechercher : Abulrob, A.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | EGFR protein, mouse; epidermal growth factor receptor; monoclonal antibody; quantum dot; brain tumor; fluorescence microscopy; glioblastoma; infrared radiation; metabolism; methodology; molecular imaging; mouse; nude mouse; pathology; reproducibility; sensitivity and specificity; tumor cell line; Antibodies, Monoclonal; Brain Neoplasms; Cell Line, Tumor; Glioblastoma; Infrared Rays; Mice; Mice, Nude; Microscopy, Fluorescence; Molecular Imaging; Quantum Dots; Receptor, Epidermal Growth Factor; Reproducibility of Results; Sensitivity and Specificity |
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| Résumé | Diagnosis of glioblastoma multiform (GBM) with MRI lacks molecular information and requires a biopsy for pathologic confirmation. The EGFRvIII, is a constitutively active mutant of the EGF receptor, identified in a high percentage of brain cancers and associated with increased invasiveness and resistance, making it a good target to improve imaging and diagnosis. The present study shows that conjugation of near-infrared quantum dot (Qd800) to an anti-EGFRvIII single domain antibody, made of the variable region with an extra cysteine for site-specific conjugation (EG2-Cys), increased its internalization in U87MG-EGFRvIII cells in vitro compared to Qd800 conjugated with the Fc region of the antibody (EG2-hFc) or unconjugated. EG2-Cys also improved the contrast in Near-Infrared Imaging of mice bearing orthotopic glioblastoma. The increased accumulation was confirmed by fluorescence microscopy of brain sections. The specificity of EG2-Cys in brain tumor expressing the EGFRvIII mutant receptor may provide an accurate less invasive diagnosis and determine the level of tumor aggressiveness and resistance. Copyright © 2014 American Scientific Publishers All rights reserved. |
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| Date de publication | 2014 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC-HHT-53273 |
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| Numéro NPARC | 21272265 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | a5e45cec-5d76-4f0a-b0d3-a5823f1821ff |
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| Enregistrement créé | 2014-07-23 |
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| Enregistrement modifié | 2020-04-22 |
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