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- Voir les données supplémentaires : Comparison of the zebrafish embryo toxicity assay and the general and behavioral embryo toxicity assay as new approach methods for chemical screening (PDF, 227 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.3390/toxics8040126 |
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| Auteur | Rechercher : Achenbach, John C.1; Rechercher : Leggiadro, Cindy1; Rechercher : Sperker, Sandra A.1; Rechercher : Woodland, Cindy; Rechercher : Ellis, Lee D.1 |
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| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Article |
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| Sujet | zebrafish larvae; toxicity assay; phenotype; developmental period |
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| Résumé | The movement away from mammalian testing of potential toxicants and new chemical entities has primarily led to cell line testing and protein-based assays. However, these assays may not yet be sufficient to properly characterize the toxic potential of a chemical. The zebrafish embryo model is widely recognized as a potential new approach method for chemical testing that may provide a bridge between cell and protein-based assays and mammalian testing. The Zebrafish Embryo Toxicity (ZET) model is increasingly recognized as a valuable toxicity testing platform. The ZET assay focuses on the early stages of embryo development and is considered a more humane model compared to adult zebrafish testing. A complementary model has been developed that exposes larvae to toxicants at a later time point during development where body patterning has already been established. Here we compare the toxicity profiles of 20 compounds for this General and Behavioral Toxicity (GBT) assay to the ZET assay. The results show partially overlapping toxicity profiles along with unique information provided by each assay. It appears from this work that these two assays applied together can strengthen the use of zebrafish embryos/larvae as standard toxicity testing models. |
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| Date de publication | 2020-12-21 |
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| Maison d’édition | MDPI AG |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | b19280f1-9ebf-47f5-af6e-eef3c6b03450 |
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| Enregistrement créé | 2021-04-15 |
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| Enregistrement modifié | 2021-04-20 |
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