| DOI | Trouver le DOI : https://doi.org/10.1007/s00299-020-02556-6 |
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| Auteur | Rechercher : Zaidi, Mohsin Abbas; Rechercher : O'Leary, Stephen J. B.1; Rechercher : Gagnon, Christine; Rechercher : Chabot, Denise; Rechercher : Wu, Shaobo; Rechercher : Hubbard, Keith; Rechercher : Tran, Frances; Rechercher : Sprott, Dave; Rechercher : Hassan, Dhuha; Rechercher : Vucurevich, Tara; Rechercher : Sheedy, Claudia; Rechercher : Laroche, André; Rechercher : Gleddie, Steve; Rechercher : Robert, Laurian S.Identifiant ORCID : https://orcid.org/0000-0003-1416-7525 |
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| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Article |
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| Sujet | triticale; Brachypodium; liquid transfer protein; nsLTP; pollen; tapetum |
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| Résumé | Two anther-expressed non-specific lipid transfer proteins (nsLTPs) were identified in triticale (× Triticosecale Wittmack). LTPc3a and LTPc3b contain a putative signal peptide sequence and eight cysteine residues in a C-Xn-C-Xn-CC-Xn-CXC-Xn-C-Xn-C pattern. These proteins belong to the type III class of nsLTPs which are expressed exclusively in the inflorescence of angiosperms. The level of LTPc3 transcript in the anther was highest at the tetrad and uninucleate microspore stages, and absent in mature pollen. In situ hybridization showed that LTPc3 was expressed in the tapetal layer of the developing triticale anther. The expression of the LTPc3 protein peaked at the uninucleate microspore stage, but was also found to be associated with the mature pollen. Accordingly, an LTPc3a::GFP translational fusion expressed in transgenic Brachypodium distachyon first showed activity in the tapetum, then in the anther locule, and later on the mature pollen grain. Altogether, these results represent the first detailed characterization of a Triticeae anther-expressed type III nsLTP with possible roles in pollen cell wall formation. |
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| Date de publication | 2020-07-07 |
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| Maison d’édition | Springer |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC-ACRD-PDB043 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | b543e6c8-7abd-4097-86e1-411edf8deb3d |
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| Enregistrement créé | 2020-08-11 |
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| Enregistrement modifié | 2020-08-11 |
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