| Téléchargement | - Voir la version finale : Snapshot of proteomic changes in Aspergillus oryzae during various stages of fermentative processing of pea protein isolate (PDF, 3.4 Mio)
- Voir les données supplémentaires partie 1 : Snapshot of proteomic changes in Aspergillus oryzae during various stages of fermentative processing of pea protein isolate (PPTX, 1.4 Mio)
- Voir les données supplémentaires partie 2 : Snapshot of proteomic changes in Aspergillus oryzae during various stages of fermentative processing of pea protein isolate (XLSX, 165 Kio)
- Voir les données supplémentaires partie 3 : Snapshot of proteomic changes in Aspergillus oryzae during various stages of fermentative processing of pea protein isolate (XLSX, 44 Kio)
|
|---|
| DOI | Trouver le DOI : https://doi.org/10.1016/j.fochms.2023.100169 |
|---|
| Auteur | Rechercher : Das, Prem Prakash1; Rechercher : Xu, Caishuang1; Rechercher : Lu, Yuping1; Rechercher : Khorsandi, Azita; Rechercher : Tanaka, Takuji; Rechercher : Korber, Darren; Rechercher : Nickerson, Michael; Rechercher : Rajagopalan, Nandhakishore1 |
|---|
| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
|
|---|
| Bailleur de fonds | Rechercher : National Research Council Canada |
|---|
| Format | Texte, Article |
|---|
| Sujet | Pisum sativum; Aspergillus oryzae; solid-state fermentation; pea protein isolate; methionine synthase; pea proteomics |
|---|
| Résumé | Pea (Pisum sativum) is one of the most abundant and sustainable alternate source of protein. Although pea proteins have good quantities of most of the essential amino acids, they have a limited supply of tryptophan, methionine and cysteine. Moreover, pea proteins have poor techno-functional properties compared to proteins from animal sources, limiting their use in certain food applications. Bioprocessing techniques like solid-state fermentation (SSF) and enzymatic processing have been explored to improve the nutrient profile and functionality of pea proteins. However, there is a lack of information about proteomic changes in the food matrix during fermentation of the pea substrate. In this research, samples during SSF of pea protein isolate with Aspergillus oryzae were used for shotgun mass spectrometry (LC-MS/MS) analysis to identify the underlying functional pathways which play direct or indirect roles in enabling the colonization of the substrate leading to potential improvement of functional and nutritional value of pea protein. Results revealed the identity of A. oryzae proteins involved in different metabolic pathways that differed during various stages of SSF. Among them, methionine synthase was identified as an abundant protein, which catalyzes methionine biosynthesis. This might suggest how fermentation processes could be used to improve the presence of sulfur containing amino acids to rebalance the essential amino acid profile and improve the nutritional quality of pea proteins. |
|---|
| Date de publication | 2023-03-01 |
|---|
| Maison d’édition | Elsevier |
|---|
| Licence | |
|---|
| Dans | |
|---|
| Langue | anglais |
|---|
| Publications évaluées par des pairs | Oui |
|---|
| Identificateur | S2666566223000096 |
|---|
| Exporter la notice | Exporter en format RIS |
|---|
| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
|---|
| Identificateur de l’enregistrement | b8128fa3-931c-4bd0-8748-45acfbb35141 |
|---|
| Enregistrement créé | 2023-08-21 |
|---|
| Enregistrement modifié | 2023-08-21 |
|---|
