| Résumé | Biobutanol is increasingly regarded as the second generation biofuel of choice. Microbial production of butanol is however still conventionally based on the utilization of carbohydrates as carbon feedstock. More and more companies are interested in using alternative carbon sources, such as biomass, notably by combining conversion of biomass into syngas via gasification and microbial fermentation of syngas components. To date, only few syngas-fermenting microorganisms that can produce butanol are known. Discovering new microorganisms or microbial consortia capable of fermenting syngas into liquid biofuels, and engineering them to make them commercially attractive is thus primordial in a strategy to develop an economically viable platform for biobutanol production. This presentation will discuss the use of next-generation sequencing technology to perform microbial community analyses of anaerobic undefined mixed cultures, with the objective to identify microbial species particularly adapted to the bioconversion of carbon monoxide (CO), a major component of syngas, into butanol |
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