| DOI | Trouver le DOI : https://doi.org/10.1104/pp.122.4.1193 |
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| Auteur | Rechercher : Periappuram, Cyril1; Rechercher : Steinhauer, Lee1; Rechercher : Barton, Dennis L.1; Rechercher : Taylor, David1; Rechercher : Chatson, Brock1; Rechercher : Zou, Jitao1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
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| Format | Texte, Article |
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| Résumé | An Arabidopsis cDNA (AtPGMp) encoding the plastidic phosphoglucomutase (PGM) predicted a 623-amino acid protein with an N-terminal sequence typical of a plastid signal peptide. Expression of a recombinant protein inEscherichia coli confirmed its enzyme activity. The recombinant enzyme had an apparent Kₘ value of 98.5 μM and a Vₘₐₓ of 4.48 μmol min⁻¹ (mg protein)⁻¹. The Calvin cycle intermediates fructose-1,6-bisphosphate and ribulose-1,5-bisphosphate exerted an inhibitory effect on PGM activity, supporting its proposed involvement in controlling photosynthetic carbon flow. A point mutation was identified in the AtPGMp gene of the Arabidopsis pgm-1 mutant. The mutation in the mutant transcript generated a stop codon at about one third of the wild-type open reading frame, and thus rendered the polypeptide nonfunctional. Storage lipid analysis of the pgm-1 mutant seeds showed a 40% reduction in oil content compared with that of wild type. Our results indicate that plastidic PGM is an important factor affecting carbon flux in triacylglycerol accumulation in oilseed plants, most likely through its essential role in starch synthesis. |
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| Date de publication | 2000-04-01 |
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| Maison d’édition | Oxford Academic
American Society of Plant Biologists |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 13052560 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | b8ea1e6d-6b54-405c-9a64-5a026a8c709d |
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| Enregistrement créé | 2009-11-25 |
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| Enregistrement modifié | 2023-04-14 |
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