| Téléchargement | - Voir le manuscrit accepté : Development of a SYBR safe TM technique for the sensitive detection of DNA in cesium chloride density gradients for stable isotope probing assays (PDF, 594 Kio)
|
|---|
| DOI | Trouver le DOI : https://doi.org/10.1016/j.mimet.2008.01.016 |
|---|
| Auteur | Rechercher : Martineau, Christine1; Rechercher : Whyte, Lyle G.; Rechercher : Greer, Charles W.1 |
|---|
| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
|
|---|
| Format | Texte, Article |
|---|
| Sujet | environmental; stable isotope probing; cesium chloride density gradient; SYBR safe™; ethidium bromide |
|---|
| Résumé | SYBR safe™, a fluorescent nucleic acid stain, was evaluated as a replacement for ethidium bromide (EtBr) in cesium chloride (CsCl) density gradients for DNA stable isotope probing (DNA-SIP) assays. The separation of ¹²C- and ¹³C-labelled DNA using SYBR safe™ gave similar results to those obtained using EtBr with pure cultures and environmental samples exposed to a ¹³C-labelled substrate, while the detection limit of DNA was enhanced by the use of SYBR safe™ by at least 5 times. The results demonstrated that SYBR safe™ is a safe, sensitive and effective alternative to the use of ethidium bromide in CsCl density gradients for DNA-SIP assays. |
|---|
| Date de publication | 2008-02-14 |
|---|
| Dans | |
|---|
| Langue | anglais |
|---|
| Numéro du CNRC | NRCC 49950 |
|---|
| Numéro NPARC | 12465408 |
|---|
| Exporter la notice | Exporter en format RIS |
|---|
| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
|---|
| Identificateur de l’enregistrement | badd1c5b-cc10-478e-9c09-46cc5f6de442 |
|---|
| Enregistrement créé | 2009-10-26 |
|---|
| Enregistrement modifié | 2020-04-15 |
|---|
