| DOI | Trouver le DOI : https://doi.org/10.1016/j.nbt.2014.04.003 |
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| Auteur | Rechercher : Py, Christophe1; Rechercher : Denhoff, Michael W.1; Rechercher : Sabourin, Nicaulas1; Rechercher : Weber, John1; Rechercher : Shiu, Matthew2; Rechercher : Zhao, Ping1 |
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| Affiliation | - Conseil national de recherches du Canada. Technologies de l'information et des communications
- Conseil national de recherches du Canada. Dispositifs médicaux
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| Format | Texte, Article |
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| Sujet | Capacitance; Plasma applications; Silicon; Air plasmas; Automated setup; Manual assembly; Neuro-chips; Patch-clamp; Planar patch clamps; Shunt capacitance; Testing time; Electrophysiology; glue; poly(methyl methacrylate); silicon; accuracy; air plasma reactor; automation; biochip; biocompatibility; bioreactor; electric potential; electrophysiology; instrument sterilization; materials testing; nanofabrication; neurochip; planar patch clamp |
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| Résumé | We report on the systematic and automated priming and testing of silicon planar patch-clamp chips after their assembly in Plexiglas packages and sterilization in an air plasma reactor. We find that almost 90% of the chips are successfully primed by our automated setup, and have a shunt capacitance of between 10. pF and 30. pF. Blocked chips are mostly due to glue invasion in the well, and variability in the manual assembly process is responsible for the distribution in shunt capacitance value. Priming and testing time with our automated setup is less than 5. min per chip, which is compatible with the production of large series for use in electrophysiology experiments. |
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| Date de publication | 2014-04-26 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21272750 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | bb6f67cd-4a0a-441d-8c79-226d4e78bbac |
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| Enregistrement créé | 2014-12-03 |
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| Enregistrement modifié | 2020-04-22 |
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