| DOI | Trouver le DOI : https://doi.org/10.1021/ja902636m |
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| Auteur | Rechercher : van der Wiel, Ingrid M.1; Rechercher : Cheng, Jenny1; Rechercher : Koukiekolo, Roger1; Rechercher : Lyn, Rodney K.1; Rechercher : Stevens, Nathan; Rechercher : O'Connor, Naphtali; Rechercher : Turro, Nicholas J.; Rechercher : Pezacki, John Paul1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
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| Format | Texte, Article |
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| Sujet | phenanthridine; fluorescein moiety; protein; FLEth; siRNA; liposomes; nucleoli |
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| Résumé | Here we report that the phenanthridine derivative covalently linked to a fluorescein moiety (FLEth) can act as a fluorescence based probe for duplex short interfering RNA (siRNA) and that this probe can also be used to report on protein−RNA interactions. A fluorescence resonance energy transfer (FRET) signal that is observed at 600 nm occurs when FLEth is complexed with siRNA. At least 2 molecules of FLEth can bind to 21 nt duplex siRNA, and the dissociation constants for these interactions are reported. We find that FLEth can also report on the interaction of siRNAs with the Carnation Italian ringspot viral suppressor of RNA silencing p19. FLEth does not bind to the siRNA−p19 complex nor can p19 bind to the siRNA−FLEth complex; rather FLEth can report on the fraction of siRNA that is unbound. FLEth can also bind siRNA in delivery systems such as liposomes. Once the siRNA reaches the interior of Huh 7.5 cells, FLEth dissociates from the siRNA and is found in the nucleoli suggesting that FLEth cannot bind to siRNAs that are associated with the RNA silencing machinery. |
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| Date de publication | 2009 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Publication du CNRC | Cette publication n’est pas du CNRCCe sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC. |
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| Numéro NPARC | 16080397 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | bbb1777b-33ae-422a-ad61-a5b2e103b429 |
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| Enregistrement créé | 2010-09-21 |
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| Enregistrement modifié | 2020-04-16 |
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