| DOI | Trouver le DOI : https://doi.org/10.2478/v10048-009-0018-2 |
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| Auteur | Rechercher : Sowa, Michael G.1; Rechercher : Mostaco-Guidolin, Leila B.1; Rechercher : Smith, Michael S. D.1; Rechercher : Kohlenberg, Elica K.1; Rechercher : Ridsdale, Andrew2; Rechercher : Stolow, Albert2; Rechercher : Ko, Alex C. T.2 |
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| Affiliation | - Conseil national de recherches du Canada. Institut du biodiagnostic du CNRC
- Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
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| Format | Texte, Article |
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| Sujet | Nonlinear optical microscopy, coherent anti-Stokes Raman scattering, two photon excited fluorescence, second harmonic generation, atherosclerosis |
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| Résumé | Nonlinear optical (NLO) microscopy is used to follow key structural and biochemical changes associated with the progression of atherosclerosis. Arteries from WHHL-MI rabbits are examined using a 3 channel NLO microscope that can simultaneously monitor the coherent anti-stokes Raman scattered light (CARS), the two-photon excited fluorescence (TPEF) and the second harmonic generation (SHG) from a sample. Distinct differences in the nonlinear optical signals are observed that correlate with the age of the vessel and the presence of atherosclerotic plaque. These differences are attributed to the changing extracellular matrix and the increased lipid deposition associated with plaque development. The capability of NLO to perform 3D sectioning in thick highly scattering vessels in order to visualize structural details of the artery wall and highlight vessel pathology is demonstrated. These features make NLO a potentially valuable tool to help understand the progression of atherosclerosis. |
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| Date de publication | 2009-09-03 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 19926385 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | bc9904d4-f91d-4614-8616-caa91eadd3eb |
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| Enregistrement créé | 2012-05-10 |
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| Enregistrement modifié | 2020-04-16 |
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