| DOI | Trouver le DOI : https://doi.org/10.1128/EC.00387-06 |
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| Auteur | Rechercher : Dignard, Daniel1; Rechercher : El-Naggar, Ahmed L.; Rechercher : Logue, Mary E.; Rechercher : Butler, Geraldine; Rechercher : Whiteway, Malcolm1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | acids; amino acids; biotechnology; candida; Candida albicans; cells; genes; genome; genome sequence; pharmaceutical; protein; proteins; Saccharomyces cerevisiae |
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| Résumé | In the opaque state, MTLa and MTLalpha strains of Candida albicans are able to mate, and this mating is directed by a pheromone-mediated signaling process. We have used comparisons of genome sequences to identify a C. albicans gene encoding a candidate a-specific mating factor. This gene is conserved in Candida dubliniensis and is similar to a three-gene family in the related fungus Candida parapsilosis but has extremely limited similarity to the Saccharomyces cerevisiae MFA1 (ScMFA1) and ScMFA2 genes. All these genes encode C-terminal CAAX box motifs characteristic of prenylated proteins. The C. albicans gene, designated CaMFA1, is found on chromosome 2 between ORF19.2165 and ORF19.2219. MFA1 encodes an open reading frame of 42 amino acids that is predicted to be processed to a 14-amino-acid prenylated mature pheromone. Microarray analysis shows that MFA1 is poorly expressed in opaque MTLa cells but is induced when the cells are treated with alpha-factor. Disruption of this C. albicans gene blocks the mating of MTLa cells but not MTLalpha cells, while the reintegration of the gene suppresses this cell-type-specific mating defect |
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| Date de publication | 2007 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | 47551 |
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| Numéro NPARC | 3540322 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | bc9aa966-150c-4113-8891-8fbd8499068a |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-05-10 |
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