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Structural characterization of the extracellular polysaccharide from Vibrio cholerae O1 El-Tor

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1371/journal.pone.0086751
AuteurRechercher : ; Rechercher : ; Rechercher : ; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Thérapeutique en santé humaine
FormatTexte, Article
Sujetexopolysaccharide; glucosamine; glycine; glycoconjugate; hydrochloric acid; n acetylglucosamine; biofilm; carbohydrate analysis; chemical binding; chemical modification; extracellular matrix; isolation procedure; proton nuclear magnetic resonance; structure activity relation; structure analysis; Vibrio cholerae; viscosity
Résumé
The ability to form biofilms is important for environmental survival, transmission, and infectivity of Vibrio cholerae, the causative agent of cholera in humans. To form biofilms, V. cholerae produces an extracellular matrix composed of proteins, nucleic acids and a glycoconjugate, termed Vibrio exopolysaccharide (VPS). Here, we present the data on isolation and characterization of the polysaccharide part of the VPS (VPS-PS), which has the following structure: -4)-α-GulpNAcAGly3OAc-(1-4)-β-D-Glcp-(1-4)- α-Glcp-(1-4)-α-D-Galp-(1- where α-D-Glc is partially (∼20%) replaced with α-D-GlcNAc. α-GulNAcAGly is an amide between 2-acetamido-2-deoxy-α-guluronic acid and glycine. Apparently, the polysaccharide is bound to a yet unidentified component, which gives it high viscosity and completely suppresses any NMR signals belonging to the sugar chains of the VPS. The only reliable method to remove this component at present is a treatment of the whole glycoconjugate with concentrated hydrochloric acid.
Date de publication
Maison d’éditionPLOS
Dans
  • PLoS ONE 9, nº 1, e86751.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21272635
Exporter la noticeExporter en format RIS
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Identificateur de l’enregistrementbeb13b06-1d70-4430-9d37-4f2418909926
Enregistrement créé2014-12-03
Enregistrement modifié2021-09-17
Date de modification :