| DOI | Trouver le DOI : https://doi.org/10.1021/la301332e |
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| Auteur | Rechercher : Weeraman, Champika1; Rechercher : Chen, Maohui1; Rechercher : Moffatt, Douglas J.1; Rechercher : Lausten, Rune1; Rechercher : Stolow, Albert1; Rechercher : Johnston, Linda J.1 |
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| Affiliation | - Conseil national de recherches du Canada. Technologies de sécurité et de rupture
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| Format | Texte, Article |
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| Résumé | A combination of vibrational sum frequency generation spectroscopy and atomic force microscopy is used to study the changes in morphology and conformational order in monolayers prepared from three natural sphingomyelin (SM) mixtures as a function of surface pressure and cholesterol concentration. The most homogeneous SM gave monolayers with well-ordered acyl chains and few gauche defects with relatively small effects of either increasing surface pressure or cholesterol addition. Heterogeneous SM mixtures with a mixture of acyl chain lengths or with significant fractions of unsaturated acyl chains had much larger contributions from gauche defects at low surface pressure and gave increasingly well-ordered monolayers as the surface pressure increased. They also showed substantial increases in lipid chain order after cholesterol addition. Overall, these results are consistent with the strong hydrogen bonding capacity of SM leading to well-ordered monolayers over a range of surface pressures. The changes in acyl chain order for natural SMs as a function of cholesterol are relevant to formation of sphingolipid–cholesterol enriched domains in cell membranes. |
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| Date de publication | 2012-08-13 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21268902 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | c0cf24dd-44f5-4cb9-834e-08a1727b427b |
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| Enregistrement créé | 2013-11-25 |
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| Enregistrement modifié | 2020-04-21 |
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