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Evaluation of a cotyledonary node regeneration system for Agrobacterium-mediated transformation of pea (Pisum sativum L.)

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/BF02632256
AuteurRechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Sujettransformation; pea; Pisum sativum; Agrobacterium tumefaciens; regeneration; progeny testing
Résumé
A rapid regeneration system was used for studies of Agrobacterium-mediated transformation in Pisum sativum L. Cotyledonary node explants were inoculated with Agrobacterium tumefaciens strains containing binary vectors carrying genes for nopaline synthase (NOS),β-glucuronidase (GUS), and neomycin phosphotransferase (NPTII) and placed on selection medium containing either 75 or 150 mg/liter kanamycin. A GUS encoding gene (uidA) containing an intron was used to monitor gene expression from 6 to 21 days postinoculation. GUS activity could be observed 6 days after inoculation in the area of the explant in which regeneration-occurred. Regenerating tissue containing transformed cells was observed in explants on selection medium 21 days postinoculation. Using this system, a single transgenic plant was obtained. Progeny of this plant, which contained two T-DNA inserts, demonstrated segregation for the inserts and for expression of the NOS gene in the selfed R₁ progeny. NPTII activity was observed in the R2 generation, indicating inheritance and expression of the foreign DNA over at least two generations. Attempts to repeat this procedure were unsuccessful.
Date de publication
Maison d’éditionSpringer Nature
Dans
  • In Vitro Cellular & Developmental Biology - Plant 29, nº 2 (avril 1993) : 77–82.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurBF02632256
NRCC-35897
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrementc50b16fa-91f7-4224-ab47-ebb5b328f18b
Enregistrement créé2023-11-06
Enregistrement modifié2023-11-06
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